Localization of the enantiozymes of 6-hydroxy-nicotine oxidase in Arthrobacter oxidans by electron immunochemistry.
نویسندگان
چکیده
During the course of growth of Arthrobacter oxidans, induction of the enantiozymes 6-hydroxy-D-nicotine oxidase and 6-hydroxy-L-nicotine oxidase occurred in the presence of DL-nicotine. Cryoultramicrotomed sections obtained from cells grown to stationary phase were gold immunolabeled. The results obtained demonstrate that both enzymes are localized in the cytoplasm.
منابع مشابه
Covalently bound flavin in D-6-hydroxynicotine oxidase from Arthrobacter oxidans. Purification and properties of D-6-hydroxynicotine oxidase.
oxidase 3 . Moreover, methylene blue was shown to be a strong inhibitor of the overall reaction. Oneelectron acceptors are also ineffective with the Dspecific enzyme; however, methylene blue and 2.6dichlorophenol-indophenol are able to reoxidize the reduced D-6-hydroxynicotine oxidase. In the presence of these dyes the rates of the overall process are higher than those with oxygen. Anaerobic ti...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 163 2 شماره
صفحات -
تاریخ انتشار 1985